other:
Recombinant:Article Title: Slow AMPA receptors in hippocampal principal cells
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Tetrodotoxin (TTX) Tocris Cat#1078 D-AP5 Tocris Cat#0106 Gabazine Tocris Cat1262 UBP-310 Tocris Cat# 3621 NBQX disodium salt HelloBio Cat#HB0443 GYKI 52466 AbCam Cat#ab146716 NASPM trihydrochloride Bio-techne Cat# 2766 Alexa Fluor-594 Hydrazide ThermoFisher Cat#A10438 (+)-MK 801 maleate AbCam Cat#ab120027 CGP 55845 Hydrochloride Sigma Aldrich Cat#SML0594 (RS)-MCGP Tocris Cat#0336 MNI-caged-L-Glutamate HelloBio Cat#HB0423 Experimental models: Cell lines HEK293 cells Leibniz Institute German Collection of Microorganisms and Cell Cultures CVCL_0045 Experimental models: Organisms/strains Mouse C57BL/6J Jackson Laboratory N/A Recombinant DNA g-8-DsRed-Max-N1 pRK5 Roger Nicoll, Carbone and Plested (2016) N/A Null g-8 (g2-in-g8-chim1-short-ECS2) DsRed Max N-1 pRK5 Riva et al. (2017) N/A Null g-2 (Stg-ECS1-del-ECS2-mut) DsRed Max-N1 pRK5 Riva et al. (2017) N/A Software and algorithms Axograph Axograph SCR_014284 pClamp Molecular Devices SCR_011323 IGOR Pro 8 WaveMetrics RRID: SCR_000325 Fiji ImageJ RRID: SCR_003070 Excel Microsoft RRID: SCR_016137 SysCon Rapp Optoelectronic - ..
Article Title: Mu-opioid receptor activation potentiates excitatory transmission at the habenulo-peduncular synapse.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-DsRed Takara Bio Cat# 632496; RRID: AB_10013483 Alexa Fluor 594 AffiniPure Donkey AntiRabbit IgG Jackson ImmunoResearch Cat# 711-585-152; RRID: AB_2340621 Chicken anti-RFP Rockland Cat# 600-901-379; RRID: AB_10704808 Alexa Fluor 594 AffiniPure Donkey AntiChicken IgG Jackson ImmunoResearch Cat# 703-585-155; RRID: AB_2340377 Bacterial and virus strains AAV5-EF1a-DIO-hChR2(H134R)-mCherry Addgene RRID: Addgene_20297 AAV5-EF1a-DIO-hChR2(H134R)-EYFP Addgene RRID: Addgene_20298 AAV5-hSyn-Cre Addgene RRID: Addgene_105553 Chemicals, peptides, and recombinant proteins DAMGO Tocris Cat# 1171 Naloxone hydrochloride Tocris Cat# 0599 DNQX Tocris Cat# 2312 DL-AP5 Tocris Cat# 3693 Gabazine Tocris Cat# 1262 CGP-55845 hydrochloride Tocris Cat# 1248 Tetrodotoxin (TTX) Tocris Cat# 1069 4-aminopyridine (4-AP) Tocris Cat# 0940 Picrotoxin (PTX) Sigma-Aldrich Cat# P1675 Baclofen Tocris Cat# 0796 Atropine Sigma-Aldrich Cat# PHR3846 LY-341495 Tocris Cat# 1209 QX-314 bromide Tocris Cat# 1014 Experimental models: Organisms/strains Mouse: Oprm1 (MOR)Cre/Cre: C57BL/6Oprm1tm2.1(EGFP/cre)Ics/KffJ Bailly et al.54 RRID:IMSR_JAX:038053 Mouse: Wild-type (WT): C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:000664 Mouse: Ai6: B6.Cg-Gt(ROSA) 26Sortm6(CAG-ZsGreen1)Hze/J The Jackson Laboratory RRID:IMSR_JAX:007906 Mouse: Ai14: B6.Cg-Gt(ROSA) 26Sortm14(CAG-tdTomato)Hze/J The Jackson Laboratory RRID:IMSR_JAX:007914 Mouse: Ai27D: B6.Cg-Gt(ROSA) 26Sortm27.1(CAG-COP4*H134R/tdTomato)Hze/J The Jackson Laboratory RRID:IMSR_JAX:012567 Mouse: Ai32: B6.Cg-Gt(ROSA) 26Sortm32(CAG-COP4*H134R/EYFP)Hze/J The Jackson Laboratory RRID:IMSR_JAX:024109 Mouse: ChatCre/Cre: B6;129S6Chattm2(cre)Lowl/J The Jackson Laboratory RRID:IMSR_JAX:006410 Mouse: Oprm1fl/fl: B6;129-Oprm1tm1.1Cgrf/ KffJ The Jackson Laboratory RRID:IMSR_JAX:030074 Software and algorithms pClamp software v10 Molecular Devices RRID:SCR_011323 Zen 2 Blue Zeiss RRID:SCR_013672 GraphPad Prism v10 GraphPad Software RRID:SCR_002798 Inkscape Inkscape RRID:SCR_014479 (Continued on next page) 12 Cell Reports 44, 115874, July 22, 2025 ..
Article Title: Mono-methylation of lysine 27 at histone 3 confers lifelong susceptibility to stress.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-H3K27me1 - CUT&RUN-seq Active Motif Cat#61015; RRID:AB_2715573 Anti-H3K27me1 - Immunofluorescence, RNAscope Millipore Sigma Cat#07-448; RRID:AB_310623 Anti-H3K27me2 - CUT&RUN-seq, Immunofluorescence, RNAscope Cell Signaling Technology Cat#9728; RRID:AB_1281338 Anti-H3K27me3 - Immunofluorescence Cell Signaling Technology Cat#9733; RRID:AB_2616029 IgG - CUT&RUN-seq Cell Signaling Technology Cat#2729; RRID:AB_1031062 Anti-GFP - Immunofluorescence Aves Labs Cat#GFP-1010; RRID:AB_2307313 Anti-SUZ-12 - Western blot Abcam Cat#ab126577; RRID:AB_11159280 Anti-EZH2- Western blot Thermo Fisher Scientific Cat#36-6300; RRID:AB_2533271 Anti-EED - Western blot Cell Signaling Technology Cat#85322; RRID:AB_2923355 Anti-TATA-binding protein (TBP) - Western blot Abcam Cat#mAbcam 51841; RRID:AB_945758 Bacterial and virus strains AAV9-hSyn-DIO-VEFS-eGFP UMB Virus Vector Core N/A AAV9-hSyn-DIO-SUZ-12-eGFP UMB Virus Vector Core N/A AAV9-hSyn-DIO-eGFP UMB Virus Vector Core N/A pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Plasmid #44361; RRID:Addgene_44361 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Addgene Plasmid #44362; RRID:Addgene_44362 pAAV-hSyn-DIO-mCherry Addgene Plasmid #50459; RRID:Addgene_50459 Chemicals, peptides, and recombinant proteins Ketamine Vedco NDC 50989-996-06 Xylazine AnaSed NDC14043-700-50 Tetradotoxin Tocris Cat1078 Gabazine Tocris Cat#1262 CNQX Tocris Cat#1045 Pentobarbital Sodium Vortech Cat#9373 Clozapine N-oxide Tocris Bioscience Cat#4936 BioMagPlus Concanavalin A beads Polysciences Cat#86057-3 SYBR green Applied Biosystems Cat#A25741 RNase-Free DNase Set (50) Qiagen Cat#79254 Microcystin Enzo Biochem Cat#ALX-350-012 Micrococcal nuclease EpiCypher Cat#15-1016 Trypsin Promega Cat#VA9000 2-methylbutane Fisher Scientific Cat#M32631 Co-Detection Target Retrieval Solution Advanced Cell Diagnostics Cat#323165 8-16% SDS-PAGE Bio-Rad Cat#4561103 0.5 mM EDTA, pH 8.0 solution ThermoFisher Cat#AM9260G UltraPure (1M) Tris-HCI, pH 8.0 ThermoFisher Cat#15568025 Blocker BSA ThermoFisher Cat#37520 RNase A, DNase and protease-free ThermoFisher Cat#EN0531 UltraPure Phenol:Chloroform:Isoamyl Alcohol ThermoFisher Cat#15593031 Proteinase K ThermoFisher Cat#EO0491 KCl (2M), RNase-free ThermoFisher Cat#AM9640G NaCl (5M), RNase-free ThermoFisher Cat#AM9760G (Continued on next page) e1 Neuron 112, 1–17.e1–e10, September 4, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER MgCl2 (1M) ThermoFisher Cat#AM9530G Triton X-100 solution Sigma-Aldrich Cat#93443 Glycogen Sigma-Aldrich Cat#10901393001 Complete EDTA-free protease inhibitor Sigma-Aldrich Cat#11873580001 Spermidine Sigma-Aldrich Cat#S2501 Complete, Mini, EDTA-free Protease Inhibitor Sigma-Aldrich Cat#11836170001 DL-Dithiothreitol solution Sigma-Aldrich Cat#646563 HEPES potassium salt Sigma-Aldrich Cat#H0527 HEPES sodium salt solution Sigma-Aldrich Cat#H3662 Trichloroacetic acid Sigma-Aldrich Cat#T0699 Saccharin Sigma-Aldrich Cat#109185 AEBSF solution Sigma-Aldrich Cat#SBR00015 Sodium butyrate Sigma-Aldrich Cat#B5887 IGEPAL Sigma-Aldrich Cat#I8896 Manganese (II) chloride solution Sigma-Aldrich Cat#M1787 Calcium chloride solution Sigma-Aldrich Cat#21115 Critical commercial assays NEBNext Ultra II DNA Library Prep Kit New England Biolabs Cat#E7645 NEBNext Multiplex Oligos for Illumina - Dual Index Primers Set 1 New England Biolabs Cat#E7600S SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian Takara Bio USA Cat#634487 SMARTer RNA Unique Dual Index Kit – 96U Set A Takara Bio USA Cat# 634452 Multiplex Fluorescent Reagent Kit v2 protocol Advanced Cell Diagnostics Cat#323100 SNAP-ChIP K-MetStat Panel EpiCypher Cat#19-1001 NucleoSpin Gel and PCR Clean-Up Kit Macherey-Nagel Cat#740609 miRNeasy Micro Kit Qiagen Cat#217084 RNeasy Mini Kit Qiagen Cat# 74104 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4374967 High Sensitivity DNA Bioanalyzer assay Agilent Cat#5067-4626 Qubit dsDNA HS assay kit Thermo Scientific Cat#Q32854 High Sensitivity D1000 ScreenTape Agilent Cat#5067-5584 High Sensitivity RNA ScreenTape analysis Agilent Cat#5067-5579 Deposited data Raw and analyzed data - CUT&RUN adult study This paper GEO: GSE233419 Raw and analyzed data - CUT&RUN ELS study This paper GEO: GSE233419 Raw and analyzed data – RNA-seq AAV-VEFS study This paper GEO: GSE233419 Raw and analyzed data - CUT&RUN AAV-VEFS study This paper GEO: GSE233419 Experimental models: Organisms/strains Mouse: C57BL/6 wild-type mice The Jackson Laboratory N/A Mouse: Drd1a-Cre hemizygote bacterial artificial chromosome transgenic The Jackson Laboratory Line FK150 Mouse: ERa-Cre Esr1-Cre knockin mice The Jackson Laboratory Line: 017911 Mouse: CD-1 retired breeder Charles River Laboratories N/A Oligonucleotides Primers for the VEFS-BOX subunit: Forward: 5’-GGA TCC TGA ATG GCT AAG AG-3’ Reverse: 5’-TAC AAA CAG CAT ACA GGC AT-3’ This paper N/A (Continued on next page) Neuron 112, 1–17.e1–e10, September 4, 2024 e2
Article Title: Spinal sensory neurons project onto the hindbrain to stabilize posture and enhance locomotor speed.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins a-Bungarotoxin Tocris Cat# 2133 MS-222 Sigma-Aldrich Cat# A5040 N-Phenylthiourea (PTU) Sigma-Aldrich Cat# P7629 D-AP5 Tocris Cat# 0106 CNQX Tocris Cat# 0190/10 Gabazine Tocris Cat# SR95531 AlexaFluor 647 Hydrazide Thermo Fisher Scientific Cat# A-20502 Biocytine Alexa Fluor 594 Thermo Fisher Scientific Cat#A12922 Sulforhodamine B sodium salt (rhodamine) Sigma-Aldrich Cat# S1402 Deposited data CSFcN_ablation_Escape.mat Wyart lab https://github.com/MingYuedanio/ Wu-et-al-2020 CSFcN_ablation_Exploratory.mat Wyart lab https://github.com/MingYuedanio/ Wu-et-al-2020 Experimental models: Organisms/strains ZebraFish: Tg(pkd2l1:GAL4)icm10Tg 45 ZFIN: ZDB-ALT-150324-1 ZebraFish: Tg(a-actin:GAL4)icm51 This study N/A ZebraFish: Tg(UAS:ChR2-mCherry)s1985t 105 ZFIN: ZDB-ALT-101227-5 ZebraFish: Tg(UAS:ChR2-YFP)icm11Tg 45 ZFIN: ZDB-ALT-150324-2 ZebraFish: Tg(UAS:GFP) 107 N/A ZebraFish: Tg(pkd2l1:TagRFP)icm17Tg 37 ZFIN: ZDB-ALT-160119-8 ZebraFish: Tg(pkd2l1:GFP)icm56Tg This study N/A ZebraFish: Tg(parg:GFP)mn2EtTg 64 ZFIN: ZDB-FISH-150901-28475 ZebraFish: Tg(Tol056:GFP)zf206EtTg 80 ZFIN: ZDB-ALT-110217-6 ZebraFish: Tg(vmat2:GFP)zf710Tg 88 ZFIN: ZDB-ALT-170608-1 ZebraFish: Tg(zCREST2hsp70:GFP) rw011aTg 65 ZFIN: ZDB-ALT-050414-10 ZebraFish: Tg(glyt2:loxP-mCherry-loxPGFP)nns23Tg 87 ZFIN: ZDB-ALT-131127-4 ZebraFish: Tg(vglut2a:loxP-DsRed-loxPGFP)zf139Tg 86 ZFIN: ZDB-ALT-090716-1 ZebraFish: Tg(vsx2:GFP)nns1Tg 83 ZFIN: ZDB-ALT-061204-2 ZebraFish: TgBAC(vsx2:GAL4FF)nns18Tg 106 ZFIN: ZDB-ALT-130617-1 ZebraFish: Tg(gad1b:GFP)nns25 87 ZFIN: ZDB-ALT-131127-6 Construct UAS:synaptophysin-mCherry This study N/A pT2MUAS:mCherry-zGephyrin-aP1 Gift from Prof. Hiromi Hirata N/A Software and algorithms Fiji 110 https://fiji.sc/; RRID: SCR_002285 MATLAB MathWorks https://www.mathworks.com/ Clampfit 10.7 Molecular Devices https://www.moleculardevices.com/ R, version 3.5.2 The R project for statistical computing112 http://cran.rproject.org/ GraphPad Prism 8.0.2 GraphPad Software https://www.graphpad.com/ ZebraZoom 91 https://zebrazoom.org/ MobileNet V2 Google111 https://tfhub.dev/ ImageNet Google https://tfhub.dev/ e1 Current Biology 31, 3315–3329.e1–e5, August 9, 2021 ll ..
Article Title: Hippocampal-evoked inhibition of cholinergic interneurons in the nucleus accumbens.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains AAV1-CaMKIIa-ChR2-mCherry Addgene 26975-AAV1 AAV9-CAG-DIO-ChroME.ST-p2A-H2BmRuby3 Addgene 108912-AAV9 AAV1-EF1a-DIO-EYFP Penn Vector Core AV-1-27056 AAV9-FLEX-ArchT-GFP UNC Vector Core N/A AAV1-EF1a-FLEX-TVA-Cherry UNC Vector Core N/A AAV9-CAG-FLEX-oG Salk N/A SADDG-GFP(EnvA) Salk N/A Chemicals, peptides, and recombinant proteins Biocytin Sigma-Aldrich Cat # B1758 Streptavidin-647 Invitrogen Cat # S21374 CPP Tocris Cat # 0247 TTX Tocris Cat # 1069 4-AP Tocris Cat # 0940 NBQX Tocris Cat # 1044 Gabazine Tocris Cat # 1262 TEA Tocris Cat # 3068 QX-314 Tocris Cat # 2313 VectaShield with DAPI Vector Labs RRID: AB_2336790 EGTA Fluka Cat # 03379 HEPES Sigma-Aldrich Cat # H3375 Experimental models: Organisms/strains Mouse: ChAT-Cre Jackson Lab RRID: IMSR_JAX:006410 Mouse: ChAT-eGFP Jackson Lab RRID: IMSR_JAX_007902 Mouse: C57BL/6J Jackson Lab RRID: IMSR_JAX:000664 Mouse: D1-tdTomato Jackson Lab RRID: IMSR_JAX:016204 Mouse: PV-2A-Cre Jackson Lab RRID: IMSR_JAX:012358 Mouse: SOM-Cre Jackson Lab RRID: IMSR_JAX:013044 Software and algorithms Igor Pro WaveMetrics RRID: SCR_000325 MATLAB MathWorks RRID: SCR_001622 Prism GraphPad Software RRID: SCR_002798 ..
Article Title: Optimized Design and in Vivo Application of Optogenetically Modified Drosophila Dopamine Receptors to Elucidate Endogenous Functions
Article Snippet: .. ©@ CelPress STAR* METHODS KEY RESOURCES TABLE Neuron REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains rAAV2/18&2.CamKlla(0.4).OPN3-mScarlet This paper N/A rAAV2/1&2.CamKlla..eYFP.WPRE This paper N/A rAAV2/1&2.CamKla(0.4).eOPN3-mScarlet This paper www.addgene.org/125712/ rAAV2/1&2.hSyn.SIO-eOPN3-mScarlet This paper www.addgene.org/125713/ Chemicals, peptides, and recombinant proteins (R)-baclofen Tocris Cat#0796 Clozapine-N-Oxide Enzo Life Science Cat#-BML-NS105 CPPene Tocris Cat#1265 Gabazine Tocris Cat#1262 NBQX Tocris Cat#1044 Pertussis toxin Sigma-Aldrich Cat#516560 Picrotoxin Tocris Cat#1128 SCH23390 Tocris Cat#0925 Critical commercial assays GloSensor cAMP Assay Promega Cat#E1171 Experimental models: Cell lines HEK293T Sigma-Aldrich Cat#12022001 RRID:CVCL_0063 Experimental models: Organisms/strains Mouse: C57BL/6JRecHsd Envigo Cat#043 Mouse: C57BL/6NHsd Envigo Cat#044 Mouse: DAT-IRES-Cre The Jackson Laboratory Strain #006660 Rattus norvegicus: Sprague-Dawley Envigo Cat#002 Rattus norvegicus: Wistar Charles River, bred in the Cat#003 animal facility, UKE Hamburg Recombinant DNA pAAV-CaMkKlla(0.4)-OPN3-mScarlet This Paper N/A pAAV-CaMkKlla(0.4)-PufTMT3a-mScarlet This Paper N/A pAAV-CaMKlla(0.4)-OPN3-M4-mScarlet This Paper N/A pAAV-CaMKlla(0.4)-PufTMT3a-M4-mScarlet This Paper N/A pAAV-CamKlla-eYFP. pcDNA3.1-GIRK2-1 pcDNA3.1-mCerulean pAAV-CaMKlla(0.4)-eOPN3-mScarlet pAAV-hSyn-SIO-eOPN3-mScarlet Software and algorithms Fiji MATLAB 2018b Prism 8.2.1 RStudio Desktop Ephus. .. WaveSurfer e1 Neuron 109, 1621-1635.e1-e8, May 19, 2021 Karl Deisseroth Eitan Reuveny Dave Piston; Rizzo et al.,2004 This Paper This Paper Schindelin et al., 2012 Mathworks Graphpad RStudio Suter et al., 2010 Janelia 117 RRID:Addgene_105622; www.addgene.org/105622 GenBank: NM_001025584.2 RRID:Addgene_15214; www.addgene.org/15214/ RRID:Addgene_125712; www.addgene.org/125712/ RRID:Addgene_125713; www.addgene.org/125713/ RRID:SCR_0022885; http://imagej.net/Fiji RRID:SCR_001622; www.mathworks.com RRID:SCR_002798; https://www.graphpad.com RRID:SCR_000432; https://www.rstudio.com https://doi.org/10.3389/fncir.2010.00100 https://wavesurfer.janelia.org (Continued on next page)
Software:Article Title: Slow AMPA receptors in hippocampal principal cells
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Tetrodotoxin (TTX) Tocris Cat#1078 D-AP5 Tocris Cat#0106 Gabazine Tocris Cat1262 UBP-310 Tocris Cat# 3621 NBQX disodium salt HelloBio Cat#HB0443 GYKI 52466 AbCam Cat#ab146716 NASPM trihydrochloride Bio-techne Cat# 2766 Alexa Fluor-594 Hydrazide ThermoFisher Cat#A10438 (+)-MK 801 maleate AbCam Cat#ab120027 CGP 55845 Hydrochloride Sigma Aldrich Cat#SML0594 (RS)-MCGP Tocris Cat#0336 MNI-caged-L-Glutamate HelloBio Cat#HB0423 Experimental models: Cell lines HEK293 cells Leibniz Institute German Collection of Microorganisms and Cell Cultures CVCL_0045 Experimental models: Organisms/strains Mouse C57BL/6J Jackson Laboratory N/A Recombinant DNA g-8-DsRed-Max-N1 pRK5 Roger Nicoll, Carbone and Plested (2016) N/A Null g-8 (g2-in-g8-chim1-short-ECS2) DsRed Max N-1 pRK5 Riva et al. (2017) N/A Null g-2 (Stg-ECS1-del-ECS2-mut) DsRed Max-N1 pRK5 Riva et al. (2017) N/A Software and algorithms Axograph Axograph SCR_014284 pClamp Molecular Devices SCR_011323 IGOR Pro 8 WaveMetrics RRID: SCR_000325 Fiji ImageJ RRID: SCR_003070 Excel Microsoft RRID: SCR_016137 SysCon Rapp Optoelectronic - ..
Article Title: Mu-opioid receptor activation potentiates excitatory transmission at the habenulo-peduncular synapse.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-DsRed Takara Bio Cat# 632496; RRID: AB_10013483 Alexa Fluor 594 AffiniPure Donkey AntiRabbit IgG Jackson ImmunoResearch Cat# 711-585-152; RRID: AB_2340621 Chicken anti-RFP Rockland Cat# 600-901-379; RRID: AB_10704808 Alexa Fluor 594 AffiniPure Donkey AntiChicken IgG Jackson ImmunoResearch Cat# 703-585-155; RRID: AB_2340377 Bacterial and virus strains AAV5-EF1a-DIO-hChR2(H134R)-mCherry Addgene RRID: Addgene_20297 AAV5-EF1a-DIO-hChR2(H134R)-EYFP Addgene RRID: Addgene_20298 AAV5-hSyn-Cre Addgene RRID: Addgene_105553 Chemicals, peptides, and recombinant proteins DAMGO Tocris Cat# 1171 Naloxone hydrochloride Tocris Cat# 0599 DNQX Tocris Cat# 2312 DL-AP5 Tocris Cat# 3693 Gabazine Tocris Cat# 1262 CGP-55845 hydrochloride Tocris Cat# 1248 Tetrodotoxin (TTX) Tocris Cat# 1069 4-aminopyridine (4-AP) Tocris Cat# 0940 Picrotoxin (PTX) Sigma-Aldrich Cat# P1675 Baclofen Tocris Cat# 0796 Atropine Sigma-Aldrich Cat# PHR3846 LY-341495 Tocris Cat# 1209 QX-314 bromide Tocris Cat# 1014 Experimental models: Organisms/strains Mouse: Oprm1 (MOR)Cre/Cre: C57BL/6Oprm1tm2.1(EGFP/cre)Ics/KffJ Bailly et al.54 RRID:IMSR_JAX:038053 Mouse: Wild-type (WT): C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:000664 Mouse: Ai6: B6.Cg-Gt(ROSA) 26Sortm6(CAG-ZsGreen1)Hze/J The Jackson Laboratory RRID:IMSR_JAX:007906 Mouse: Ai14: B6.Cg-Gt(ROSA) 26Sortm14(CAG-tdTomato)Hze/J The Jackson Laboratory RRID:IMSR_JAX:007914 Mouse: Ai27D: B6.Cg-Gt(ROSA) 26Sortm27.1(CAG-COP4*H134R/tdTomato)Hze/J The Jackson Laboratory RRID:IMSR_JAX:012567 Mouse: Ai32: B6.Cg-Gt(ROSA) 26Sortm32(CAG-COP4*H134R/EYFP)Hze/J The Jackson Laboratory RRID:IMSR_JAX:024109 Mouse: ChatCre/Cre: B6;129S6Chattm2(cre)Lowl/J The Jackson Laboratory RRID:IMSR_JAX:006410 Mouse: Oprm1fl/fl: B6;129-Oprm1tm1.1Cgrf/ KffJ The Jackson Laboratory RRID:IMSR_JAX:030074 Software and algorithms pClamp software v10 Molecular Devices RRID:SCR_011323 Zen 2 Blue Zeiss RRID:SCR_013672 GraphPad Prism v10 GraphPad Software RRID:SCR_002798 Inkscape Inkscape RRID:SCR_014479 (Continued on next page) 12 Cell Reports 44, 115874, July 22, 2025 ..
Article Title: Spinal sensory neurons project onto the hindbrain to stabilize posture and enhance locomotor speed.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins a-Bungarotoxin Tocris Cat# 2133 MS-222 Sigma-Aldrich Cat# A5040 N-Phenylthiourea (PTU) Sigma-Aldrich Cat# P7629 D-AP5 Tocris Cat# 0106 CNQX Tocris Cat# 0190/10 Gabazine Tocris Cat# SR95531 AlexaFluor 647 Hydrazide Thermo Fisher Scientific Cat# A-20502 Biocytine Alexa Fluor 594 Thermo Fisher Scientific Cat#A12922 Sulforhodamine B sodium salt (rhodamine) Sigma-Aldrich Cat# S1402 Deposited data CSFcN_ablation_Escape.mat Wyart lab https://github.com/MingYuedanio/ Wu-et-al-2020 CSFcN_ablation_Exploratory.mat Wyart lab https://github.com/MingYuedanio/ Wu-et-al-2020 Experimental models: Organisms/strains ZebraFish: Tg(pkd2l1:GAL4)icm10Tg 45 ZFIN: ZDB-ALT-150324-1 ZebraFish: Tg(a-actin:GAL4)icm51 This study N/A ZebraFish: Tg(UAS:ChR2-mCherry)s1985t 105 ZFIN: ZDB-ALT-101227-5 ZebraFish: Tg(UAS:ChR2-YFP)icm11Tg 45 ZFIN: ZDB-ALT-150324-2 ZebraFish: Tg(UAS:GFP) 107 N/A ZebraFish: Tg(pkd2l1:TagRFP)icm17Tg 37 ZFIN: ZDB-ALT-160119-8 ZebraFish: Tg(pkd2l1:GFP)icm56Tg This study N/A ZebraFish: Tg(parg:GFP)mn2EtTg 64 ZFIN: ZDB-FISH-150901-28475 ZebraFish: Tg(Tol056:GFP)zf206EtTg 80 ZFIN: ZDB-ALT-110217-6 ZebraFish: Tg(vmat2:GFP)zf710Tg 88 ZFIN: ZDB-ALT-170608-1 ZebraFish: Tg(zCREST2hsp70:GFP) rw011aTg 65 ZFIN: ZDB-ALT-050414-10 ZebraFish: Tg(glyt2:loxP-mCherry-loxPGFP)nns23Tg 87 ZFIN: ZDB-ALT-131127-4 ZebraFish: Tg(vglut2a:loxP-DsRed-loxPGFP)zf139Tg 86 ZFIN: ZDB-ALT-090716-1 ZebraFish: Tg(vsx2:GFP)nns1Tg 83 ZFIN: ZDB-ALT-061204-2 ZebraFish: TgBAC(vsx2:GAL4FF)nns18Tg 106 ZFIN: ZDB-ALT-130617-1 ZebraFish: Tg(gad1b:GFP)nns25 87 ZFIN: ZDB-ALT-131127-6 Construct UAS:synaptophysin-mCherry This study N/A pT2MUAS:mCherry-zGephyrin-aP1 Gift from Prof. Hiromi Hirata N/A Software and algorithms Fiji 110 https://fiji.sc/; RRID: SCR_002285 MATLAB MathWorks https://www.mathworks.com/ Clampfit 10.7 Molecular Devices https://www.moleculardevices.com/ R, version 3.5.2 The R project for statistical computing112 http://cran.rproject.org/ GraphPad Prism 8.0.2 GraphPad Software https://www.graphpad.com/ ZebraZoom 91 https://zebrazoom.org/ MobileNet V2 Google111 https://tfhub.dev/ ImageNet Google https://tfhub.dev/ e1 Current Biology 31, 3315–3329.e1–e5, August 9, 2021 ll ..
Article Title: Optimized Design and in Vivo Application of Optogenetically Modified Drosophila Dopamine Receptors to Elucidate Endogenous Functions
Article Snippet: .. ©@ CelPress STAR* METHODS KEY RESOURCES TABLE Neuron REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains rAAV2/18&2.CamKlla(0.4).OPN3-mScarlet This paper N/A rAAV2/1&2.CamKlla..eYFP.WPRE This paper N/A rAAV2/1&2.CamKla(0.4).eOPN3-mScarlet This paper www.addgene.org/125712/ rAAV2/1&2.hSyn.SIO-eOPN3-mScarlet This paper www.addgene.org/125713/ Chemicals, peptides, and recombinant proteins (R)-baclofen Tocris Cat#0796 Clozapine-N-Oxide Enzo Life Science Cat#-BML-NS105 CPPene Tocris Cat#1265 Gabazine Tocris Cat#1262 NBQX Tocris Cat#1044 Pertussis toxin Sigma-Aldrich Cat#516560 Picrotoxin Tocris Cat#1128 SCH23390 Tocris Cat#0925 Critical commercial assays GloSensor cAMP Assay Promega Cat#E1171 Experimental models: Cell lines HEK293T Sigma-Aldrich Cat#12022001 RRID:CVCL_0063 Experimental models: Organisms/strains Mouse: C57BL/6JRecHsd Envigo Cat#043 Mouse: C57BL/6NHsd Envigo Cat#044 Mouse: DAT-IRES-Cre The Jackson Laboratory Strain #006660 Rattus norvegicus: Sprague-Dawley Envigo Cat#002 Rattus norvegicus: Wistar Charles River, bred in the Cat#003 animal facility, UKE Hamburg Recombinant DNA pAAV-CaMkKlla(0.4)-OPN3-mScarlet This Paper N/A pAAV-CaMkKlla(0.4)-PufTMT3a-mScarlet This Paper N/A pAAV-CaMKlla(0.4)-OPN3-M4-mScarlet This Paper N/A pAAV-CaMKlla(0.4)-PufTMT3a-M4-mScarlet This Paper N/A pAAV-CamKlla-eYFP. pcDNA3.1-GIRK2-1 pcDNA3.1-mCerulean pAAV-CaMKlla(0.4)-eOPN3-mScarlet pAAV-hSyn-SIO-eOPN3-mScarlet Software and algorithms Fiji MATLAB 2018b Prism 8.2.1 RStudio Desktop Ephus. .. WaveSurfer e1 Neuron 109, 1621-1635.e1-e8, May 19, 2021 Karl Deisseroth Eitan Reuveny Dave Piston; Rizzo et al.,2004 This Paper This Paper Schindelin et al., 2012 Mathworks Graphpad RStudio Suter et al., 2010 Janelia 117 RRID:Addgene_105622; www.addgene.org/105622 GenBank: NM_001025584.2 RRID:Addgene_15214; www.addgene.org/15214/ RRID:Addgene_125712; www.addgene.org/125712/ RRID:Addgene_125713; www.addgene.org/125713/ RRID:SCR_0022885; http://imagej.net/Fiji RRID:SCR_001622; www.mathworks.com RRID:SCR_002798; https://www.graphpad.com RRID:SCR_000432; https://www.rstudio.com https://doi.org/10.3389/fncir.2010.00100 https://wavesurfer.janelia.org (Continued on next page)
Virus:Article Title: Mu-opioid receptor activation potentiates excitatory transmission at the habenulo-peduncular synapse.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-DsRed Takara Bio Cat# 632496; RRID: AB_10013483 Alexa Fluor 594 AffiniPure Donkey AntiRabbit IgG Jackson ImmunoResearch Cat# 711-585-152; RRID: AB_2340621 Chicken anti-RFP Rockland Cat# 600-901-379; RRID: AB_10704808 Alexa Fluor 594 AffiniPure Donkey AntiChicken IgG Jackson ImmunoResearch Cat# 703-585-155; RRID: AB_2340377 Bacterial and virus strains AAV5-EF1a-DIO-hChR2(H134R)-mCherry Addgene RRID: Addgene_20297 AAV5-EF1a-DIO-hChR2(H134R)-EYFP Addgene RRID: Addgene_20298 AAV5-hSyn-Cre Addgene RRID: Addgene_105553 Chemicals, peptides, and recombinant proteins DAMGO Tocris Cat# 1171 Naloxone hydrochloride Tocris Cat# 0599 DNQX Tocris Cat# 2312 DL-AP5 Tocris Cat# 3693 Gabazine Tocris Cat# 1262 CGP-55845 hydrochloride Tocris Cat# 1248 Tetrodotoxin (TTX) Tocris Cat# 1069 4-aminopyridine (4-AP) Tocris Cat# 0940 Picrotoxin (PTX) Sigma-Aldrich Cat# P1675 Baclofen Tocris Cat# 0796 Atropine Sigma-Aldrich Cat# PHR3846 LY-341495 Tocris Cat# 1209 QX-314 bromide Tocris Cat# 1014 Experimental models: Organisms/strains Mouse: Oprm1 (MOR)Cre/Cre: C57BL/6Oprm1tm2.1(EGFP/cre)Ics/KffJ Bailly et al.54 RRID:IMSR_JAX:038053 Mouse: Wild-type (WT): C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:000664 Mouse: Ai6: B6.Cg-Gt(ROSA) 26Sortm6(CAG-ZsGreen1)Hze/J The Jackson Laboratory RRID:IMSR_JAX:007906 Mouse: Ai14: B6.Cg-Gt(ROSA) 26Sortm14(CAG-tdTomato)Hze/J The Jackson Laboratory RRID:IMSR_JAX:007914 Mouse: Ai27D: B6.Cg-Gt(ROSA) 26Sortm27.1(CAG-COP4*H134R/tdTomato)Hze/J The Jackson Laboratory RRID:IMSR_JAX:012567 Mouse: Ai32: B6.Cg-Gt(ROSA) 26Sortm32(CAG-COP4*H134R/EYFP)Hze/J The Jackson Laboratory RRID:IMSR_JAX:024109 Mouse: ChatCre/Cre: B6;129S6Chattm2(cre)Lowl/J The Jackson Laboratory RRID:IMSR_JAX:006410 Mouse: Oprm1fl/fl: B6;129-Oprm1tm1.1Cgrf/ KffJ The Jackson Laboratory RRID:IMSR_JAX:030074 Software and algorithms pClamp software v10 Molecular Devices RRID:SCR_011323 Zen 2 Blue Zeiss RRID:SCR_013672 GraphPad Prism v10 GraphPad Software RRID:SCR_002798 Inkscape Inkscape RRID:SCR_014479 (Continued on next page) 12 Cell Reports 44, 115874, July 22, 2025 ..
Article Title: Mono-methylation of lysine 27 at histone 3 confers lifelong susceptibility to stress.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-H3K27me1 - CUT&RUN-seq Active Motif Cat#61015; RRID:AB_2715573 Anti-H3K27me1 - Immunofluorescence, RNAscope Millipore Sigma Cat#07-448; RRID:AB_310623 Anti-H3K27me2 - CUT&RUN-seq, Immunofluorescence, RNAscope Cell Signaling Technology Cat#9728; RRID:AB_1281338 Anti-H3K27me3 - Immunofluorescence Cell Signaling Technology Cat#9733; RRID:AB_2616029 IgG - CUT&RUN-seq Cell Signaling Technology Cat#2729; RRID:AB_1031062 Anti-GFP - Immunofluorescence Aves Labs Cat#GFP-1010; RRID:AB_2307313 Anti-SUZ-12 - Western blot Abcam Cat#ab126577; RRID:AB_11159280 Anti-EZH2- Western blot Thermo Fisher Scientific Cat#36-6300; RRID:AB_2533271 Anti-EED - Western blot Cell Signaling Technology Cat#85322; RRID:AB_2923355 Anti-TATA-binding protein (TBP) - Western blot Abcam Cat#mAbcam 51841; RRID:AB_945758 Bacterial and virus strains AAV9-hSyn-DIO-VEFS-eGFP UMB Virus Vector Core N/A AAV9-hSyn-DIO-SUZ-12-eGFP UMB Virus Vector Core N/A AAV9-hSyn-DIO-eGFP UMB Virus Vector Core N/A pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Plasmid #44361; RRID:Addgene_44361 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Addgene Plasmid #44362; RRID:Addgene_44362 pAAV-hSyn-DIO-mCherry Addgene Plasmid #50459; RRID:Addgene_50459 Chemicals, peptides, and recombinant proteins Ketamine Vedco NDC 50989-996-06 Xylazine AnaSed NDC14043-700-50 Tetradotoxin Tocris Cat1078 Gabazine Tocris Cat#1262 CNQX Tocris Cat#1045 Pentobarbital Sodium Vortech Cat#9373 Clozapine N-oxide Tocris Bioscience Cat#4936 BioMagPlus Concanavalin A beads Polysciences Cat#86057-3 SYBR green Applied Biosystems Cat#A25741 RNase-Free DNase Set (50) Qiagen Cat#79254 Microcystin Enzo Biochem Cat#ALX-350-012 Micrococcal nuclease EpiCypher Cat#15-1016 Trypsin Promega Cat#VA9000 2-methylbutane Fisher Scientific Cat#M32631 Co-Detection Target Retrieval Solution Advanced Cell Diagnostics Cat#323165 8-16% SDS-PAGE Bio-Rad Cat#4561103 0.5 mM EDTA, pH 8.0 solution ThermoFisher Cat#AM9260G UltraPure (1M) Tris-HCI, pH 8.0 ThermoFisher Cat#15568025 Blocker BSA ThermoFisher Cat#37520 RNase A, DNase and protease-free ThermoFisher Cat#EN0531 UltraPure Phenol:Chloroform:Isoamyl Alcohol ThermoFisher Cat#15593031 Proteinase K ThermoFisher Cat#EO0491 KCl (2M), RNase-free ThermoFisher Cat#AM9640G NaCl (5M), RNase-free ThermoFisher Cat#AM9760G (Continued on next page) e1 Neuron 112, 1–17.e1–e10, September 4, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER MgCl2 (1M) ThermoFisher Cat#AM9530G Triton X-100 solution Sigma-Aldrich Cat#93443 Glycogen Sigma-Aldrich Cat#10901393001 Complete EDTA-free protease inhibitor Sigma-Aldrich Cat#11873580001 Spermidine Sigma-Aldrich Cat#S2501 Complete, Mini, EDTA-free Protease Inhibitor Sigma-Aldrich Cat#11836170001 DL-Dithiothreitol solution Sigma-Aldrich Cat#646563 HEPES potassium salt Sigma-Aldrich Cat#H0527 HEPES sodium salt solution Sigma-Aldrich Cat#H3662 Trichloroacetic acid Sigma-Aldrich Cat#T0699 Saccharin Sigma-Aldrich Cat#109185 AEBSF solution Sigma-Aldrich Cat#SBR00015 Sodium butyrate Sigma-Aldrich Cat#B5887 IGEPAL Sigma-Aldrich Cat#I8896 Manganese (II) chloride solution Sigma-Aldrich Cat#M1787 Calcium chloride solution Sigma-Aldrich Cat#21115 Critical commercial assays NEBNext Ultra II DNA Library Prep Kit New England Biolabs Cat#E7645 NEBNext Multiplex Oligos for Illumina - Dual Index Primers Set 1 New England Biolabs Cat#E7600S SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian Takara Bio USA Cat#634487 SMARTer RNA Unique Dual Index Kit – 96U Set A Takara Bio USA Cat# 634452 Multiplex Fluorescent Reagent Kit v2 protocol Advanced Cell Diagnostics Cat#323100 SNAP-ChIP K-MetStat Panel EpiCypher Cat#19-1001 NucleoSpin Gel and PCR Clean-Up Kit Macherey-Nagel Cat#740609 miRNeasy Micro Kit Qiagen Cat#217084 RNeasy Mini Kit Qiagen Cat# 74104 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4374967 High Sensitivity DNA Bioanalyzer assay Agilent Cat#5067-4626 Qubit dsDNA HS assay kit Thermo Scientific Cat#Q32854 High Sensitivity D1000 ScreenTape Agilent Cat#5067-5584 High Sensitivity RNA ScreenTape analysis Agilent Cat#5067-5579 Deposited data Raw and analyzed data - CUT&RUN adult study This paper GEO: GSE233419 Raw and analyzed data - CUT&RUN ELS study This paper GEO: GSE233419 Raw and analyzed data – RNA-seq AAV-VEFS study This paper GEO: GSE233419 Raw and analyzed data - CUT&RUN AAV-VEFS study This paper GEO: GSE233419 Experimental models: Organisms/strains Mouse: C57BL/6 wild-type mice The Jackson Laboratory N/A Mouse: Drd1a-Cre hemizygote bacterial artificial chromosome transgenic The Jackson Laboratory Line FK150 Mouse: ERa-Cre Esr1-Cre knockin mice The Jackson Laboratory Line: 017911 Mouse: CD-1 retired breeder Charles River Laboratories N/A Oligonucleotides Primers for the VEFS-BOX subunit: Forward: 5’-GGA TCC TGA ATG GCT AAG AG-3’ Reverse: 5’-TAC AAA CAG CAT ACA GGC AT-3’ This paper N/A (Continued on next page) Neuron 112, 1–17.e1–e10, September 4, 2024 e2
Article Title: Hippocampal-evoked inhibition of cholinergic interneurons in the nucleus accumbens.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains AAV1-CaMKIIa-ChR2-mCherry Addgene 26975-AAV1 AAV9-CAG-DIO-ChroME.ST-p2A-H2BmRuby3 Addgene 108912-AAV9 AAV1-EF1a-DIO-EYFP Penn Vector Core AV-1-27056 AAV9-FLEX-ArchT-GFP UNC Vector Core N/A AAV1-EF1a-FLEX-TVA-Cherry UNC Vector Core N/A AAV9-CAG-FLEX-oG Salk N/A SADDG-GFP(EnvA) Salk N/A Chemicals, peptides, and recombinant proteins Biocytin Sigma-Aldrich Cat # B1758 Streptavidin-647 Invitrogen Cat # S21374 CPP Tocris Cat # 0247 TTX Tocris Cat # 1069 4-AP Tocris Cat # 0940 NBQX Tocris Cat # 1044 Gabazine Tocris Cat # 1262 TEA Tocris Cat # 3068 QX-314 Tocris Cat # 2313 VectaShield with DAPI Vector Labs RRID: AB_2336790 EGTA Fluka Cat # 03379 HEPES Sigma-Aldrich Cat # H3375 Experimental models: Organisms/strains Mouse: ChAT-Cre Jackson Lab RRID: IMSR_JAX:006410 Mouse: ChAT-eGFP Jackson Lab RRID: IMSR_JAX_007902 Mouse: C57BL/6J Jackson Lab RRID: IMSR_JAX:000664 Mouse: D1-tdTomato Jackson Lab RRID: IMSR_JAX:016204 Mouse: PV-2A-Cre Jackson Lab RRID: IMSR_JAX:012358 Mouse: SOM-Cre Jackson Lab RRID: IMSR_JAX:013044 Software and algorithms Igor Pro WaveMetrics RRID: SCR_000325 MATLAB MathWorks RRID: SCR_001622 Prism GraphPad Software RRID: SCR_002798 ..
Article Title: Optimized Design and in Vivo Application of Optogenetically Modified Drosophila Dopamine Receptors to Elucidate Endogenous Functions
Article Snippet: .. ©@ CelPress STAR* METHODS KEY RESOURCES TABLE Neuron REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains rAAV2/18&2.CamKlla(0.4).OPN3-mScarlet This paper N/A rAAV2/1&2.CamKlla..eYFP.WPRE This paper N/A rAAV2/1&2.CamKla(0.4).eOPN3-mScarlet This paper www.addgene.org/125712/ rAAV2/1&2.hSyn.SIO-eOPN3-mScarlet This paper www.addgene.org/125713/ Chemicals, peptides, and recombinant proteins (R)-baclofen Tocris Cat#0796 Clozapine-N-Oxide Enzo Life Science Cat#-BML-NS105 CPPene Tocris Cat#1265 Gabazine Tocris Cat#1262 NBQX Tocris Cat#1044 Pertussis toxin Sigma-Aldrich Cat#516560 Picrotoxin Tocris Cat#1128 SCH23390 Tocris Cat#0925 Critical commercial assays GloSensor cAMP Assay Promega Cat#E1171 Experimental models: Cell lines HEK293T Sigma-Aldrich Cat#12022001 RRID:CVCL_0063 Experimental models: Organisms/strains Mouse: C57BL/6JRecHsd Envigo Cat#043 Mouse: C57BL/6NHsd Envigo Cat#044 Mouse: DAT-IRES-Cre The Jackson Laboratory Strain #006660 Rattus norvegicus: Sprague-Dawley Envigo Cat#002 Rattus norvegicus: Wistar Charles River, bred in the Cat#003 animal facility, UKE Hamburg Recombinant DNA pAAV-CaMkKlla(0.4)-OPN3-mScarlet This Paper N/A pAAV-CaMkKlla(0.4)-PufTMT3a-mScarlet This Paper N/A pAAV-CaMKlla(0.4)-OPN3-M4-mScarlet This Paper N/A pAAV-CaMKlla(0.4)-PufTMT3a-M4-mScarlet This Paper N/A pAAV-CamKlla-eYFP. pcDNA3.1-GIRK2-1 pcDNA3.1-mCerulean pAAV-CaMKlla(0.4)-eOPN3-mScarlet pAAV-hSyn-SIO-eOPN3-mScarlet Software and algorithms Fiji MATLAB 2018b Prism 8.2.1 RStudio Desktop Ephus. .. WaveSurfer e1 Neuron 109, 1621-1635.e1-e8, May 19, 2021 Karl Deisseroth Eitan Reuveny Dave Piston; Rizzo et al.,2004 This Paper This Paper Schindelin et al., 2012 Mathworks Graphpad RStudio Suter et al., 2010 Janelia 117 RRID:Addgene_105622; www.addgene.org/105622 GenBank: NM_001025584.2 RRID:Addgene_15214; www.addgene.org/15214/ RRID:Addgene_125712; www.addgene.org/125712/ RRID:Addgene_125713; www.addgene.org/125713/ RRID:SCR_0022885; http://imagej.net/Fiji RRID:SCR_001622; www.mathworks.com RRID:SCR_002798; https://www.graphpad.com RRID:SCR_000432; https://www.rstudio.com https://doi.org/10.3389/fncir.2010.00100 https://wavesurfer.janelia.org (Continued on next page)
Immunofluorescence:Article Title: Mono-methylation of lysine 27 at histone 3 confers lifelong susceptibility to stress.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-H3K27me1 - CUT&RUN-seq Active Motif Cat#61015; RRID:AB_2715573 Anti-H3K27me1 - Immunofluorescence, RNAscope Millipore Sigma Cat#07-448; RRID:AB_310623 Anti-H3K27me2 - CUT&RUN-seq, Immunofluorescence, RNAscope Cell Signaling Technology Cat#9728; RRID:AB_1281338 Anti-H3K27me3 - Immunofluorescence Cell Signaling Technology Cat#9733; RRID:AB_2616029 IgG - CUT&RUN-seq Cell Signaling Technology Cat#2729; RRID:AB_1031062 Anti-GFP - Immunofluorescence Aves Labs Cat#GFP-1010; RRID:AB_2307313 Anti-SUZ-12 - Western blot Abcam Cat#ab126577; RRID:AB_11159280 Anti-EZH2- Western blot Thermo Fisher Scientific Cat#36-6300; RRID:AB_2533271 Anti-EED - Western blot Cell Signaling Technology Cat#85322; RRID:AB_2923355 Anti-TATA-binding protein (TBP) - Western blot Abcam Cat#mAbcam 51841; RRID:AB_945758 Bacterial and virus strains AAV9-hSyn-DIO-VEFS-eGFP UMB Virus Vector Core N/A AAV9-hSyn-DIO-SUZ-12-eGFP UMB Virus Vector Core N/A AAV9-hSyn-DIO-eGFP UMB Virus Vector Core N/A pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Plasmid #44361; RRID:Addgene_44361 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Addgene Plasmid #44362; RRID:Addgene_44362 pAAV-hSyn-DIO-mCherry Addgene Plasmid #50459; RRID:Addgene_50459 Chemicals, peptides, and recombinant proteins Ketamine Vedco NDC 50989-996-06 Xylazine AnaSed NDC14043-700-50 Tetradotoxin Tocris Cat1078 Gabazine Tocris Cat#1262 CNQX Tocris Cat#1045 Pentobarbital Sodium Vortech Cat#9373 Clozapine N-oxide Tocris Bioscience Cat#4936 BioMagPlus Concanavalin A beads Polysciences Cat#86057-3 SYBR green Applied Biosystems Cat#A25741 RNase-Free DNase Set (50) Qiagen Cat#79254 Microcystin Enzo Biochem Cat#ALX-350-012 Micrococcal nuclease EpiCypher Cat#15-1016 Trypsin Promega Cat#VA9000 2-methylbutane Fisher Scientific Cat#M32631 Co-Detection Target Retrieval Solution Advanced Cell Diagnostics Cat#323165 8-16% SDS-PAGE Bio-Rad Cat#4561103 0.5 mM EDTA, pH 8.0 solution ThermoFisher Cat#AM9260G UltraPure (1M) Tris-HCI, pH 8.0 ThermoFisher Cat#15568025 Blocker BSA ThermoFisher Cat#37520 RNase A, DNase and protease-free ThermoFisher Cat#EN0531 UltraPure Phenol:Chloroform:Isoamyl Alcohol ThermoFisher Cat#15593031 Proteinase K ThermoFisher Cat#EO0491 KCl (2M), RNase-free ThermoFisher Cat#AM9640G NaCl (5M), RNase-free ThermoFisher Cat#AM9760G (Continued on next page) e1 Neuron 112, 1–17.e1–e10, September 4, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER MgCl2 (1M) ThermoFisher Cat#AM9530G Triton X-100 solution Sigma-Aldrich Cat#93443 Glycogen Sigma-Aldrich Cat#10901393001 Complete EDTA-free protease inhibitor Sigma-Aldrich Cat#11873580001 Spermidine Sigma-Aldrich Cat#S2501 Complete, Mini, EDTA-free Protease Inhibitor Sigma-Aldrich Cat#11836170001 DL-Dithiothreitol solution Sigma-Aldrich Cat#646563 HEPES potassium salt Sigma-Aldrich Cat#H0527 HEPES sodium salt solution Sigma-Aldrich Cat#H3662 Trichloroacetic acid Sigma-Aldrich Cat#T0699 Saccharin Sigma-Aldrich Cat#109185 AEBSF solution Sigma-Aldrich Cat#SBR00015 Sodium butyrate Sigma-Aldrich Cat#B5887 IGEPAL Sigma-Aldrich Cat#I8896 Manganese (II) chloride solution Sigma-Aldrich Cat#M1787 Calcium chloride solution Sigma-Aldrich Cat#21115 Critical commercial assays NEBNext Ultra II DNA Library Prep Kit New England Biolabs Cat#E7645 NEBNext Multiplex Oligos for Illumina - Dual Index Primers Set 1 New England Biolabs Cat#E7600S SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian Takara Bio USA Cat#634487 SMARTer RNA Unique Dual Index Kit – 96U Set A Takara Bio USA Cat# 634452 Multiplex Fluorescent Reagent Kit v2 protocol Advanced Cell Diagnostics Cat#323100 SNAP-ChIP K-MetStat Panel EpiCypher Cat#19-1001 NucleoSpin Gel and PCR Clean-Up Kit Macherey-Nagel Cat#740609 miRNeasy Micro Kit Qiagen Cat#217084 RNeasy Mini Kit Qiagen Cat# 74104 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4374967 High Sensitivity DNA Bioanalyzer assay Agilent Cat#5067-4626 Qubit dsDNA HS assay kit Thermo Scientific Cat#Q32854 High Sensitivity D1000 ScreenTape Agilent Cat#5067-5584 High Sensitivity RNA ScreenTape analysis Agilent Cat#5067-5579 Deposited data Raw and analyzed data - CUT&RUN adult study This paper GEO: GSE233419 Raw and analyzed data - CUT&RUN ELS study This paper GEO: GSE233419 Raw and analyzed data – RNA-seq AAV-VEFS study This paper GEO: GSE233419 Raw and analyzed data - CUT&RUN AAV-VEFS study This paper GEO: GSE233419 Experimental models: Organisms/strains Mouse: C57BL/6 wild-type mice The Jackson Laboratory N/A Mouse: Drd1a-Cre hemizygote bacterial artificial chromosome transgenic The Jackson Laboratory Line FK150 Mouse: ERa-Cre Esr1-Cre knockin mice The Jackson Laboratory Line: 017911 Mouse: CD-1 retired breeder Charles River Laboratories N/A Oligonucleotides Primers for the VEFS-BOX subunit: Forward: 5’-GGA TCC TGA ATG GCT AAG AG-3’ Reverse: 5’-TAC AAA CAG CAT ACA GGC AT-3’ This paper N/A (Continued on next page) Neuron 112, 1–17.e1–e10, September 4, 2024 e2
RNAscope:Article Title: Mono-methylation of lysine 27 at histone 3 confers lifelong susceptibility to stress.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-H3K27me1 - CUT&RUN-seq Active Motif Cat#61015; RRID:AB_2715573 Anti-H3K27me1 - Immunofluorescence, RNAscope Millipore Sigma Cat#07-448; RRID:AB_310623 Anti-H3K27me2 - CUT&RUN-seq, Immunofluorescence, RNAscope Cell Signaling Technology Cat#9728; RRID:AB_1281338 Anti-H3K27me3 - Immunofluorescence Cell Signaling Technology Cat#9733; RRID:AB_2616029 IgG - CUT&RUN-seq Cell Signaling Technology Cat#2729; RRID:AB_1031062 Anti-GFP - Immunofluorescence Aves Labs Cat#GFP-1010; RRID:AB_2307313 Anti-SUZ-12 - Western blot Abcam Cat#ab126577; RRID:AB_11159280 Anti-EZH2- Western blot Thermo Fisher Scientific Cat#36-6300; RRID:AB_2533271 Anti-EED - Western blot Cell Signaling Technology Cat#85322; RRID:AB_2923355 Anti-TATA-binding protein (TBP) - Western blot Abcam Cat#mAbcam 51841; RRID:AB_945758 Bacterial and virus strains AAV9-hSyn-DIO-VEFS-eGFP UMB Virus Vector Core N/A AAV9-hSyn-DIO-SUZ-12-eGFP UMB Virus Vector Core N/A AAV9-hSyn-DIO-eGFP UMB Virus Vector Core N/A pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Plasmid #44361; RRID:Addgene_44361 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Addgene Plasmid #44362; RRID:Addgene_44362 pAAV-hSyn-DIO-mCherry Addgene Plasmid #50459; RRID:Addgene_50459 Chemicals, peptides, and recombinant proteins Ketamine Vedco NDC 50989-996-06 Xylazine AnaSed NDC14043-700-50 Tetradotoxin Tocris Cat1078 Gabazine Tocris Cat#1262 CNQX Tocris Cat#1045 Pentobarbital Sodium Vortech Cat#9373 Clozapine N-oxide Tocris Bioscience Cat#4936 BioMagPlus Concanavalin A beads Polysciences Cat#86057-3 SYBR green Applied Biosystems Cat#A25741 RNase-Free DNase Set (50) Qiagen Cat#79254 Microcystin Enzo Biochem Cat#ALX-350-012 Micrococcal nuclease EpiCypher Cat#15-1016 Trypsin Promega Cat#VA9000 2-methylbutane Fisher Scientific Cat#M32631 Co-Detection Target Retrieval Solution Advanced Cell Diagnostics Cat#323165 8-16% SDS-PAGE Bio-Rad Cat#4561103 0.5 mM EDTA, pH 8.0 solution ThermoFisher Cat#AM9260G UltraPure (1M) Tris-HCI, pH 8.0 ThermoFisher Cat#15568025 Blocker BSA ThermoFisher Cat#37520 RNase A, DNase and protease-free ThermoFisher Cat#EN0531 UltraPure Phenol:Chloroform:Isoamyl Alcohol ThermoFisher Cat#15593031 Proteinase K ThermoFisher Cat#EO0491 KCl (2M), RNase-free ThermoFisher Cat#AM9640G NaCl (5M), RNase-free ThermoFisher Cat#AM9760G (Continued on next page) e1 Neuron 112, 1–17.e1–e10, September 4, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER MgCl2 (1M) ThermoFisher Cat#AM9530G Triton X-100 solution Sigma-Aldrich Cat#93443 Glycogen Sigma-Aldrich Cat#10901393001 Complete EDTA-free protease inhibitor Sigma-Aldrich Cat#11873580001 Spermidine Sigma-Aldrich Cat#S2501 Complete, Mini, EDTA-free Protease Inhibitor Sigma-Aldrich Cat#11836170001 DL-Dithiothreitol solution Sigma-Aldrich Cat#646563 HEPES potassium salt Sigma-Aldrich Cat#H0527 HEPES sodium salt solution Sigma-Aldrich Cat#H3662 Trichloroacetic acid Sigma-Aldrich Cat#T0699 Saccharin Sigma-Aldrich Cat#109185 AEBSF solution Sigma-Aldrich Cat#SBR00015 Sodium butyrate Sigma-Aldrich Cat#B5887 IGEPAL Sigma-Aldrich Cat#I8896 Manganese (II) chloride solution Sigma-Aldrich Cat#M1787 Calcium chloride solution Sigma-Aldrich Cat#21115 Critical commercial assays NEBNext Ultra II DNA Library Prep Kit New England Biolabs Cat#E7645 NEBNext Multiplex Oligos for Illumina - Dual Index Primers Set 1 New England Biolabs Cat#E7600S SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian Takara Bio USA Cat#634487 SMARTer RNA Unique Dual Index Kit – 96U Set A Takara Bio USA Cat# 634452 Multiplex Fluorescent Reagent Kit v2 protocol Advanced Cell Diagnostics Cat#323100 SNAP-ChIP K-MetStat Panel EpiCypher Cat#19-1001 NucleoSpin Gel and PCR Clean-Up Kit Macherey-Nagel Cat#740609 miRNeasy Micro Kit Qiagen Cat#217084 RNeasy Mini Kit Qiagen Cat# 74104 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4374967 High Sensitivity DNA Bioanalyzer assay Agilent Cat#5067-4626 Qubit dsDNA HS assay kit Thermo Scientific Cat#Q32854 High Sensitivity D1000 ScreenTape Agilent Cat#5067-5584 High Sensitivity RNA ScreenTape analysis Agilent Cat#5067-5579 Deposited data Raw and analyzed data - CUT&RUN adult study This paper GEO: GSE233419 Raw and analyzed data - CUT&RUN ELS study This paper GEO: GSE233419 Raw and analyzed data – RNA-seq AAV-VEFS study This paper GEO: GSE233419 Raw and analyzed data - CUT&RUN AAV-VEFS study This paper GEO: GSE233419 Experimental models: Organisms/strains Mouse: C57BL/6 wild-type mice The Jackson Laboratory N/A Mouse: Drd1a-Cre hemizygote bacterial artificial chromosome transgenic The Jackson Laboratory Line FK150 Mouse: ERa-Cre Esr1-Cre knockin mice The Jackson Laboratory Line: 017911 Mouse: CD-1 retired breeder Charles River Laboratories N/A Oligonucleotides Primers for the VEFS-BOX subunit: Forward: 5’-GGA TCC TGA ATG GCT AAG AG-3’ Reverse: 5’-TAC AAA CAG CAT ACA GGC AT-3’ This paper N/A (Continued on next page) Neuron 112, 1–17.e1–e10, September 4, 2024 e2
Western Blot:Article Title: Mono-methylation of lysine 27 at histone 3 confers lifelong susceptibility to stress.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-H3K27me1 - CUT&RUN-seq Active Motif Cat#61015; RRID:AB_2715573 Anti-H3K27me1 - Immunofluorescence, RNAscope Millipore Sigma Cat#07-448; RRID:AB_310623 Anti-H3K27me2 - CUT&RUN-seq, Immunofluorescence, RNAscope Cell Signaling Technology Cat#9728; RRID:AB_1281338 Anti-H3K27me3 - Immunofluorescence Cell Signaling Technology Cat#9733; RRID:AB_2616029 IgG - CUT&RUN-seq Cell Signaling Technology Cat#2729; RRID:AB_1031062 Anti-GFP - Immunofluorescence Aves Labs Cat#GFP-1010; RRID:AB_2307313 Anti-SUZ-12 - Western blot Abcam Cat#ab126577; RRID:AB_11159280 Anti-EZH2- Western blot Thermo Fisher Scientific Cat#36-6300; RRID:AB_2533271 Anti-EED - Western blot Cell Signaling Technology Cat#85322; RRID:AB_2923355 Anti-TATA-binding protein (TBP) - Western blot Abcam Cat#mAbcam 51841; RRID:AB_945758 Bacterial and virus strains AAV9-hSyn-DIO-VEFS-eGFP UMB Virus Vector Core N/A AAV9-hSyn-DIO-SUZ-12-eGFP UMB Virus Vector Core N/A AAV9-hSyn-DIO-eGFP UMB Virus Vector Core N/A pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Plasmid #44361; RRID:Addgene_44361 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Addgene Plasmid #44362; RRID:Addgene_44362 pAAV-hSyn-DIO-mCherry Addgene Plasmid #50459; RRID:Addgene_50459 Chemicals, peptides, and recombinant proteins Ketamine Vedco NDC 50989-996-06 Xylazine AnaSed NDC14043-700-50 Tetradotoxin Tocris Cat1078 Gabazine Tocris Cat#1262 CNQX Tocris Cat#1045 Pentobarbital Sodium Vortech Cat#9373 Clozapine N-oxide Tocris Bioscience Cat#4936 BioMagPlus Concanavalin A beads Polysciences Cat#86057-3 SYBR green Applied Biosystems Cat#A25741 RNase-Free DNase Set (50) Qiagen Cat#79254 Microcystin Enzo Biochem Cat#ALX-350-012 Micrococcal nuclease EpiCypher Cat#15-1016 Trypsin Promega Cat#VA9000 2-methylbutane Fisher Scientific Cat#M32631 Co-Detection Target Retrieval Solution Advanced Cell Diagnostics Cat#323165 8-16% SDS-PAGE Bio-Rad Cat#4561103 0.5 mM EDTA, pH 8.0 solution ThermoFisher Cat#AM9260G UltraPure (1M) Tris-HCI, pH 8.0 ThermoFisher Cat#15568025 Blocker BSA ThermoFisher Cat#37520 RNase A, DNase and protease-free ThermoFisher Cat#EN0531 UltraPure Phenol:Chloroform:Isoamyl Alcohol ThermoFisher Cat#15593031 Proteinase K ThermoFisher Cat#EO0491 KCl (2M), RNase-free ThermoFisher Cat#AM9640G NaCl (5M), RNase-free ThermoFisher Cat#AM9760G (Continued on next page) e1 Neuron 112, 1–17.e1–e10, September 4, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER MgCl2 (1M) ThermoFisher Cat#AM9530G Triton X-100 solution Sigma-Aldrich Cat#93443 Glycogen Sigma-Aldrich Cat#10901393001 Complete EDTA-free protease inhibitor Sigma-Aldrich Cat#11873580001 Spermidine Sigma-Aldrich Cat#S2501 Complete, Mini, EDTA-free Protease Inhibitor Sigma-Aldrich Cat#11836170001 DL-Dithiothreitol solution Sigma-Aldrich Cat#646563 HEPES potassium salt Sigma-Aldrich Cat#H0527 HEPES sodium salt solution Sigma-Aldrich Cat#H3662 Trichloroacetic acid Sigma-Aldrich Cat#T0699 Saccharin Sigma-Aldrich Cat#109185 AEBSF solution Sigma-Aldrich Cat#SBR00015 Sodium butyrate Sigma-Aldrich Cat#B5887 IGEPAL Sigma-Aldrich Cat#I8896 Manganese (II) chloride solution Sigma-Aldrich Cat#M1787 Calcium chloride solution Sigma-Aldrich Cat#21115 Critical commercial assays NEBNext Ultra II DNA Library Prep Kit New England Biolabs Cat#E7645 NEBNext Multiplex Oligos for Illumina - Dual Index Primers Set 1 New England Biolabs Cat#E7600S SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian Takara Bio USA Cat#634487 SMARTer RNA Unique Dual Index Kit – 96U Set A Takara Bio USA Cat# 634452 Multiplex Fluorescent Reagent Kit v2 protocol Advanced Cell Diagnostics Cat#323100 SNAP-ChIP K-MetStat Panel EpiCypher Cat#19-1001 NucleoSpin Gel and PCR Clean-Up Kit Macherey-Nagel Cat#740609 miRNeasy Micro Kit Qiagen Cat#217084 RNeasy Mini Kit Qiagen Cat# 74104 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4374967 High Sensitivity DNA Bioanalyzer assay Agilent Cat#5067-4626 Qubit dsDNA HS assay kit Thermo Scientific Cat#Q32854 High Sensitivity D1000 ScreenTape Agilent Cat#5067-5584 High Sensitivity RNA ScreenTape analysis Agilent Cat#5067-5579 Deposited data Raw and analyzed data - CUT&RUN adult study This paper GEO: GSE233419 Raw and analyzed data - CUT&RUN ELS study This paper GEO: GSE233419 Raw and analyzed data – RNA-seq AAV-VEFS study This paper GEO: GSE233419 Raw and analyzed data - CUT&RUN AAV-VEFS study This paper GEO: GSE233419 Experimental models: Organisms/strains Mouse: C57BL/6 wild-type mice The Jackson Laboratory N/A Mouse: Drd1a-Cre hemizygote bacterial artificial chromosome transgenic The Jackson Laboratory Line FK150 Mouse: ERa-Cre Esr1-Cre knockin mice The Jackson Laboratory Line: 017911 Mouse: CD-1 retired breeder Charles River Laboratories N/A Oligonucleotides Primers for the VEFS-BOX subunit: Forward: 5’-GGA TCC TGA ATG GCT AAG AG-3’ Reverse: 5’-TAC AAA CAG CAT ACA GGC AT-3’ This paper N/A (Continued on next page) Neuron 112, 1–17.e1–e10, September 4, 2024 e2
Plasmid Preparation:Article Title: Mono-methylation of lysine 27 at histone 3 confers lifelong susceptibility to stress.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-H3K27me1 - CUT&RUN-seq Active Motif Cat#61015; RRID:AB_2715573 Anti-H3K27me1 - Immunofluorescence, RNAscope Millipore Sigma Cat#07-448; RRID:AB_310623 Anti-H3K27me2 - CUT&RUN-seq, Immunofluorescence, RNAscope Cell Signaling Technology Cat#9728; RRID:AB_1281338 Anti-H3K27me3 - Immunofluorescence Cell Signaling Technology Cat#9733; RRID:AB_2616029 IgG - CUT&RUN-seq Cell Signaling Technology Cat#2729; RRID:AB_1031062 Anti-GFP - Immunofluorescence Aves Labs Cat#GFP-1010; RRID:AB_2307313 Anti-SUZ-12 - Western blot Abcam Cat#ab126577; RRID:AB_11159280 Anti-EZH2- Western blot Thermo Fisher Scientific Cat#36-6300; RRID:AB_2533271 Anti-EED - Western blot Cell Signaling Technology Cat#85322; RRID:AB_2923355 Anti-TATA-binding protein (TBP) - Western blot Abcam Cat#mAbcam 51841; RRID:AB_945758 Bacterial and virus strains AAV9-hSyn-DIO-VEFS-eGFP UMB Virus Vector Core N/A AAV9-hSyn-DIO-SUZ-12-eGFP UMB Virus Vector Core N/A AAV9-hSyn-DIO-eGFP UMB Virus Vector Core N/A pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Plasmid #44361; RRID:Addgene_44361 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Addgene Plasmid #44362; RRID:Addgene_44362 pAAV-hSyn-DIO-mCherry Addgene Plasmid #50459; RRID:Addgene_50459 Chemicals, peptides, and recombinant proteins Ketamine Vedco NDC 50989-996-06 Xylazine AnaSed NDC14043-700-50 Tetradotoxin Tocris Cat1078 Gabazine Tocris Cat#1262 CNQX Tocris Cat#1045 Pentobarbital Sodium Vortech Cat#9373 Clozapine N-oxide Tocris Bioscience Cat#4936 BioMagPlus Concanavalin A beads Polysciences Cat#86057-3 SYBR green Applied Biosystems Cat#A25741 RNase-Free DNase Set (50) Qiagen Cat#79254 Microcystin Enzo Biochem Cat#ALX-350-012 Micrococcal nuclease EpiCypher Cat#15-1016 Trypsin Promega Cat#VA9000 2-methylbutane Fisher Scientific Cat#M32631 Co-Detection Target Retrieval Solution Advanced Cell Diagnostics Cat#323165 8-16% SDS-PAGE Bio-Rad Cat#4561103 0.5 mM EDTA, pH 8.0 solution ThermoFisher Cat#AM9260G UltraPure (1M) Tris-HCI, pH 8.0 ThermoFisher Cat#15568025 Blocker BSA ThermoFisher Cat#37520 RNase A, DNase and protease-free ThermoFisher Cat#EN0531 UltraPure Phenol:Chloroform:Isoamyl Alcohol ThermoFisher Cat#15593031 Proteinase K ThermoFisher Cat#EO0491 KCl (2M), RNase-free ThermoFisher Cat#AM9640G NaCl (5M), RNase-free ThermoFisher Cat#AM9760G (Continued on next page) e1 Neuron 112, 1–17.e1–e10, September 4, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER MgCl2 (1M) ThermoFisher Cat#AM9530G Triton X-100 solution Sigma-Aldrich Cat#93443 Glycogen Sigma-Aldrich Cat#10901393001 Complete EDTA-free protease inhibitor Sigma-Aldrich Cat#11873580001 Spermidine Sigma-Aldrich Cat#S2501 Complete, Mini, EDTA-free Protease Inhibitor Sigma-Aldrich Cat#11836170001 DL-Dithiothreitol solution Sigma-Aldrich Cat#646563 HEPES potassium salt Sigma-Aldrich Cat#H0527 HEPES sodium salt solution Sigma-Aldrich Cat#H3662 Trichloroacetic acid Sigma-Aldrich Cat#T0699 Saccharin Sigma-Aldrich Cat#109185 AEBSF solution Sigma-Aldrich Cat#SBR00015 Sodium butyrate Sigma-Aldrich Cat#B5887 IGEPAL Sigma-Aldrich Cat#I8896 Manganese (II) chloride solution Sigma-Aldrich Cat#M1787 Calcium chloride solution Sigma-Aldrich Cat#21115 Critical commercial assays NEBNext Ultra II DNA Library Prep Kit New England Biolabs Cat#E7645 NEBNext Multiplex Oligos for Illumina - Dual Index Primers Set 1 New England Biolabs Cat#E7600S SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian Takara Bio USA Cat#634487 SMARTer RNA Unique Dual Index Kit – 96U Set A Takara Bio USA Cat# 634452 Multiplex Fluorescent Reagent Kit v2 protocol Advanced Cell Diagnostics Cat#323100 SNAP-ChIP K-MetStat Panel EpiCypher Cat#19-1001 NucleoSpin Gel and PCR Clean-Up Kit Macherey-Nagel Cat#740609 miRNeasy Micro Kit Qiagen Cat#217084 RNeasy Mini Kit Qiagen Cat# 74104 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4374967 High Sensitivity DNA Bioanalyzer assay Agilent Cat#5067-4626 Qubit dsDNA HS assay kit Thermo Scientific Cat#Q32854 High Sensitivity D1000 ScreenTape Agilent Cat#5067-5584 High Sensitivity RNA ScreenTape analysis Agilent Cat#5067-5579 Deposited data Raw and analyzed data - CUT&RUN adult study This paper GEO: GSE233419 Raw and analyzed data - CUT&RUN ELS study This paper GEO: GSE233419 Raw and analyzed data – RNA-seq AAV-VEFS study This paper GEO: GSE233419 Raw and analyzed data - CUT&RUN AAV-VEFS study This paper GEO: GSE233419 Experimental models: Organisms/strains Mouse: C57BL/6 wild-type mice The Jackson Laboratory N/A Mouse: Drd1a-Cre hemizygote bacterial artificial chromosome transgenic The Jackson Laboratory Line FK150 Mouse: ERa-Cre Esr1-Cre knockin mice The Jackson Laboratory Line: 017911 Mouse: CD-1 retired breeder Charles River Laboratories N/A Oligonucleotides Primers for the VEFS-BOX subunit: Forward: 5’-GGA TCC TGA ATG GCT AAG AG-3’ Reverse: 5’-TAC AAA CAG CAT ACA GGC AT-3’ This paper N/A (Continued on next page) Neuron 112, 1–17.e1–e10, September 4, 2024 e2
SYBR Green Assay:Article Title: Mono-methylation of lysine 27 at histone 3 confers lifelong susceptibility to stress.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-H3K27me1 - CUT&RUN-seq Active Motif Cat#61015; RRID:AB_2715573 Anti-H3K27me1 - Immunofluorescence, RNAscope Millipore Sigma Cat#07-448; RRID:AB_310623 Anti-H3K27me2 - CUT&RUN-seq, Immunofluorescence, RNAscope Cell Signaling Technology Cat#9728; RRID:AB_1281338 Anti-H3K27me3 - Immunofluorescence Cell Signaling Technology Cat#9733; RRID:AB_2616029 IgG - CUT&RUN-seq Cell Signaling Technology Cat#2729; RRID:AB_1031062 Anti-GFP - Immunofluorescence Aves Labs Cat#GFP-1010; RRID:AB_2307313 Anti-SUZ-12 - Western blot Abcam Cat#ab126577; RRID:AB_11159280 Anti-EZH2- Western blot Thermo Fisher Scientific Cat#36-6300; RRID:AB_2533271 Anti-EED - Western blot Cell Signaling Technology Cat#85322; RRID:AB_2923355 Anti-TATA-binding protein (TBP) - Western blot Abcam Cat#mAbcam 51841; RRID:AB_945758 Bacterial and virus strains AAV9-hSyn-DIO-VEFS-eGFP UMB Virus Vector Core N/A AAV9-hSyn-DIO-SUZ-12-eGFP UMB Virus Vector Core N/A AAV9-hSyn-DIO-eGFP UMB Virus Vector Core N/A pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Plasmid #44361; RRID:Addgene_44361 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Addgene Plasmid #44362; RRID:Addgene_44362 pAAV-hSyn-DIO-mCherry Addgene Plasmid #50459; RRID:Addgene_50459 Chemicals, peptides, and recombinant proteins Ketamine Vedco NDC 50989-996-06 Xylazine AnaSed NDC14043-700-50 Tetradotoxin Tocris Cat1078 Gabazine Tocris Cat#1262 CNQX Tocris Cat#1045 Pentobarbital Sodium Vortech Cat#9373 Clozapine N-oxide Tocris Bioscience Cat#4936 BioMagPlus Concanavalin A beads Polysciences Cat#86057-3 SYBR green Applied Biosystems Cat#A25741 RNase-Free DNase Set (50) Qiagen Cat#79254 Microcystin Enzo Biochem Cat#ALX-350-012 Micrococcal nuclease EpiCypher Cat#15-1016 Trypsin Promega Cat#VA9000 2-methylbutane Fisher Scientific Cat#M32631 Co-Detection Target Retrieval Solution Advanced Cell Diagnostics Cat#323165 8-16% SDS-PAGE Bio-Rad Cat#4561103 0.5 mM EDTA, pH 8.0 solution ThermoFisher Cat#AM9260G UltraPure (1M) Tris-HCI, pH 8.0 ThermoFisher Cat#15568025 Blocker BSA ThermoFisher Cat#37520 RNase A, DNase and protease-free ThermoFisher Cat#EN0531 UltraPure Phenol:Chloroform:Isoamyl Alcohol ThermoFisher Cat#15593031 Proteinase K ThermoFisher Cat#EO0491 KCl (2M), RNase-free ThermoFisher Cat#AM9640G NaCl (5M), RNase-free ThermoFisher Cat#AM9760G (Continued on next page) e1 Neuron 112, 1–17.e1–e10, September 4, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER MgCl2 (1M) ThermoFisher Cat#AM9530G Triton X-100 solution Sigma-Aldrich Cat#93443 Glycogen Sigma-Aldrich Cat#10901393001 Complete EDTA-free protease inhibitor Sigma-Aldrich Cat#11873580001 Spermidine Sigma-Aldrich Cat#S2501 Complete, Mini, EDTA-free Protease Inhibitor Sigma-Aldrich Cat#11836170001 DL-Dithiothreitol solution Sigma-Aldrich Cat#646563 HEPES potassium salt Sigma-Aldrich Cat#H0527 HEPES sodium salt solution Sigma-Aldrich Cat#H3662 Trichloroacetic acid Sigma-Aldrich Cat#T0699 Saccharin Sigma-Aldrich Cat#109185 AEBSF solution Sigma-Aldrich Cat#SBR00015 Sodium butyrate Sigma-Aldrich Cat#B5887 IGEPAL Sigma-Aldrich Cat#I8896 Manganese (II) chloride solution Sigma-Aldrich Cat#M1787 Calcium chloride solution Sigma-Aldrich Cat#21115 Critical commercial assays NEBNext Ultra II DNA Library Prep Kit New England Biolabs Cat#E7645 NEBNext Multiplex Oligos for Illumina - Dual Index Primers Set 1 New England Biolabs Cat#E7600S SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian Takara Bio USA Cat#634487 SMARTer RNA Unique Dual Index Kit – 96U Set A Takara Bio USA Cat# 634452 Multiplex Fluorescent Reagent Kit v2 protocol Advanced Cell Diagnostics Cat#323100 SNAP-ChIP K-MetStat Panel EpiCypher Cat#19-1001 NucleoSpin Gel and PCR Clean-Up Kit Macherey-Nagel Cat#740609 miRNeasy Micro Kit Qiagen Cat#217084 RNeasy Mini Kit Qiagen Cat# 74104 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4374967 High Sensitivity DNA Bioanalyzer assay Agilent Cat#5067-4626 Qubit dsDNA HS assay kit Thermo Scientific Cat#Q32854 High Sensitivity D1000 ScreenTape Agilent Cat#5067-5584 High Sensitivity RNA ScreenTape analysis Agilent Cat#5067-5579 Deposited data Raw and analyzed data - CUT&RUN adult study This paper GEO: GSE233419 Raw and analyzed data - CUT&RUN ELS study This paper GEO: GSE233419 Raw and analyzed data – RNA-seq AAV-VEFS study This paper GEO: GSE233419 Raw and analyzed data - CUT&RUN AAV-VEFS study This paper GEO: GSE233419 Experimental models: Organisms/strains Mouse: C57BL/6 wild-type mice The Jackson Laboratory N/A Mouse: Drd1a-Cre hemizygote bacterial artificial chromosome transgenic The Jackson Laboratory Line FK150 Mouse: ERa-Cre Esr1-Cre knockin mice The Jackson Laboratory Line: 017911 Mouse: CD-1 retired breeder Charles River Laboratories N/A Oligonucleotides Primers for the VEFS-BOX subunit: Forward: 5’-GGA TCC TGA ATG GCT AAG AG-3’ Reverse: 5’-TAC AAA CAG CAT ACA GGC AT-3’ This paper N/A (Continued on next page) Neuron 112, 1–17.e1–e10, September 4, 2024 e2
SDS Page:Article Title: Mono-methylation of lysine 27 at histone 3 confers lifelong susceptibility to stress.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-H3K27me1 - CUT&RUN-seq Active Motif Cat#61015; RRID:AB_2715573 Anti-H3K27me1 - Immunofluorescence, RNAscope Millipore Sigma Cat#07-448; RRID:AB_310623 Anti-H3K27me2 - CUT&RUN-seq, Immunofluorescence, RNAscope Cell Signaling Technology Cat#9728; RRID:AB_1281338 Anti-H3K27me3 - Immunofluorescence Cell Signaling Technology Cat#9733; RRID:AB_2616029 IgG - CUT&RUN-seq Cell Signaling Technology Cat#2729; RRID:AB_1031062 Anti-GFP - Immunofluorescence Aves Labs Cat#GFP-1010; RRID:AB_2307313 Anti-SUZ-12 - Western blot Abcam Cat#ab126577; RRID:AB_11159280 Anti-EZH2- Western blot Thermo Fisher Scientific Cat#36-6300; RRID:AB_2533271 Anti-EED - Western blot Cell Signaling Technology Cat#85322; RRID:AB_2923355 Anti-TATA-binding protein (TBP) - Western blot Abcam Cat#mAbcam 51841; RRID:AB_945758 Bacterial and virus strains AAV9-hSyn-DIO-VEFS-eGFP UMB Virus Vector Core N/A AAV9-hSyn-DIO-SUZ-12-eGFP UMB Virus Vector Core N/A AAV9-hSyn-DIO-eGFP UMB Virus Vector Core N/A pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Plasmid #44361; RRID:Addgene_44361 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Addgene Plasmid #44362; RRID:Addgene_44362 pAAV-hSyn-DIO-mCherry Addgene Plasmid #50459; RRID:Addgene_50459 Chemicals, peptides, and recombinant proteins Ketamine Vedco NDC 50989-996-06 Xylazine AnaSed NDC14043-700-50 Tetradotoxin Tocris Cat1078 Gabazine Tocris Cat#1262 CNQX Tocris Cat#1045 Pentobarbital Sodium Vortech Cat#9373 Clozapine N-oxide Tocris Bioscience Cat#4936 BioMagPlus Concanavalin A beads Polysciences Cat#86057-3 SYBR green Applied Biosystems Cat#A25741 RNase-Free DNase Set (50) Qiagen Cat#79254 Microcystin Enzo Biochem Cat#ALX-350-012 Micrococcal nuclease EpiCypher Cat#15-1016 Trypsin Promega Cat#VA9000 2-methylbutane Fisher Scientific Cat#M32631 Co-Detection Target Retrieval Solution Advanced Cell Diagnostics Cat#323165 8-16% SDS-PAGE Bio-Rad Cat#4561103 0.5 mM EDTA, pH 8.0 solution ThermoFisher Cat#AM9260G UltraPure (1M) Tris-HCI, pH 8.0 ThermoFisher Cat#15568025 Blocker BSA ThermoFisher Cat#37520 RNase A, DNase and protease-free ThermoFisher Cat#EN0531 UltraPure Phenol:Chloroform:Isoamyl Alcohol ThermoFisher Cat#15593031 Proteinase K ThermoFisher Cat#EO0491 KCl (2M), RNase-free ThermoFisher Cat#AM9640G NaCl (5M), RNase-free ThermoFisher Cat#AM9760G (Continued on next page) e1 Neuron 112, 1–17.e1–e10, September 4, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER MgCl2 (1M) ThermoFisher Cat#AM9530G Triton X-100 solution Sigma-Aldrich Cat#93443 Glycogen Sigma-Aldrich Cat#10901393001 Complete EDTA-free protease inhibitor Sigma-Aldrich Cat#11873580001 Spermidine Sigma-Aldrich Cat#S2501 Complete, Mini, EDTA-free Protease Inhibitor Sigma-Aldrich Cat#11836170001 DL-Dithiothreitol solution Sigma-Aldrich Cat#646563 HEPES potassium salt Sigma-Aldrich Cat#H0527 HEPES sodium salt solution Sigma-Aldrich Cat#H3662 Trichloroacetic acid Sigma-Aldrich Cat#T0699 Saccharin Sigma-Aldrich Cat#109185 AEBSF solution Sigma-Aldrich Cat#SBR00015 Sodium butyrate Sigma-Aldrich Cat#B5887 IGEPAL Sigma-Aldrich Cat#I8896 Manganese (II) chloride solution Sigma-Aldrich Cat#M1787 Calcium chloride solution Sigma-Aldrich Cat#21115 Critical commercial assays NEBNext Ultra II DNA Library Prep Kit New England Biolabs Cat#E7645 NEBNext Multiplex Oligos for Illumina - Dual Index Primers Set 1 New England Biolabs Cat#E7600S SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian Takara Bio USA Cat#634487 SMARTer RNA Unique Dual Index Kit – 96U Set A Takara Bio USA Cat# 634452 Multiplex Fluorescent Reagent Kit v2 protocol Advanced Cell Diagnostics Cat#323100 SNAP-ChIP K-MetStat Panel EpiCypher Cat#19-1001 NucleoSpin Gel and PCR Clean-Up Kit Macherey-Nagel Cat#740609 miRNeasy Micro Kit Qiagen Cat#217084 RNeasy Mini Kit Qiagen Cat# 74104 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4374967 High Sensitivity DNA Bioanalyzer assay Agilent Cat#5067-4626 Qubit dsDNA HS assay kit Thermo Scientific Cat#Q32854 High Sensitivity D1000 ScreenTape Agilent Cat#5067-5584 High Sensitivity RNA ScreenTape analysis Agilent Cat#5067-5579 Deposited data Raw and analyzed data - CUT&RUN adult study This paper GEO: GSE233419 Raw and analyzed data - CUT&RUN ELS study This paper GEO: GSE233419 Raw and analyzed data – RNA-seq AAV-VEFS study This paper GEO: GSE233419 Raw and analyzed data - CUT&RUN AAV-VEFS study This paper GEO: GSE233419 Experimental models: Organisms/strains Mouse: C57BL/6 wild-type mice The Jackson Laboratory N/A Mouse: Drd1a-Cre hemizygote bacterial artificial chromosome transgenic The Jackson Laboratory Line FK150 Mouse: ERa-Cre Esr1-Cre knockin mice The Jackson Laboratory Line: 017911 Mouse: CD-1 retired breeder Charles River Laboratories N/A Oligonucleotides Primers for the VEFS-BOX subunit: Forward: 5’-GGA TCC TGA ATG GCT AAG AG-3’ Reverse: 5’-TAC AAA CAG CAT ACA GGC AT-3’ This paper N/A (Continued on next page) Neuron 112, 1–17.e1–e10, September 4, 2024 e2
Construct:Article Title: Spinal sensory neurons project onto the hindbrain to stabilize posture and enhance locomotor speed.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins a-Bungarotoxin Tocris Cat# 2133 MS-222 Sigma-Aldrich Cat# A5040 N-Phenylthiourea (PTU) Sigma-Aldrich Cat# P7629 D-AP5 Tocris Cat# 0106 CNQX Tocris Cat# 0190/10 Gabazine Tocris Cat# SR95531 AlexaFluor 647 Hydrazide Thermo Fisher Scientific Cat# A-20502 Biocytine Alexa Fluor 594 Thermo Fisher Scientific Cat#A12922 Sulforhodamine B sodium salt (rhodamine) Sigma-Aldrich Cat# S1402 Deposited data CSFcN_ablation_Escape.mat Wyart lab https://github.com/MingYuedanio/ Wu-et-al-2020 CSFcN_ablation_Exploratory.mat Wyart lab https://github.com/MingYuedanio/ Wu-et-al-2020 Experimental models: Organisms/strains ZebraFish: Tg(pkd2l1:GAL4)icm10Tg 45 ZFIN: ZDB-ALT-150324-1 ZebraFish: Tg(a-actin:GAL4)icm51 This study N/A ZebraFish: Tg(UAS:ChR2-mCherry)s1985t 105 ZFIN: ZDB-ALT-101227-5 ZebraFish: Tg(UAS:ChR2-YFP)icm11Tg 45 ZFIN: ZDB-ALT-150324-2 ZebraFish: Tg(UAS:GFP) 107 N/A ZebraFish: Tg(pkd2l1:TagRFP)icm17Tg 37 ZFIN: ZDB-ALT-160119-8 ZebraFish: Tg(pkd2l1:GFP)icm56Tg This study N/A ZebraFish: Tg(parg:GFP)mn2EtTg 64 ZFIN: ZDB-FISH-150901-28475 ZebraFish: Tg(Tol056:GFP)zf206EtTg 80 ZFIN: ZDB-ALT-110217-6 ZebraFish: Tg(vmat2:GFP)zf710Tg 88 ZFIN: ZDB-ALT-170608-1 ZebraFish: Tg(zCREST2hsp70:GFP) rw011aTg 65 ZFIN: ZDB-ALT-050414-10 ZebraFish: Tg(glyt2:loxP-mCherry-loxPGFP)nns23Tg 87 ZFIN: ZDB-ALT-131127-4 ZebraFish: Tg(vglut2a:loxP-DsRed-loxPGFP)zf139Tg 86 ZFIN: ZDB-ALT-090716-1 ZebraFish: Tg(vsx2:GFP)nns1Tg 83 ZFIN: ZDB-ALT-061204-2 ZebraFish: TgBAC(vsx2:GAL4FF)nns18Tg 106 ZFIN: ZDB-ALT-130617-1 ZebraFish: Tg(gad1b:GFP)nns25 87 ZFIN: ZDB-ALT-131127-6 Construct UAS:synaptophysin-mCherry This study N/A pT2MUAS:mCherry-zGephyrin-aP1 Gift from Prof. Hiromi Hirata N/A Software and algorithms Fiji 110 https://fiji.sc/; RRID: SCR_002285 MATLAB MathWorks https://www.mathworks.com/ Clampfit 10.7 Molecular Devices https://www.moleculardevices.com/ R, version 3.5.2 The R project for statistical computing112 http://cran.rproject.org/ GraphPad Prism 8.0.2 GraphPad Software https://www.graphpad.com/ ZebraZoom 91 https://zebrazoom.org/ MobileNet V2 Google111 https://tfhub.dev/ ImageNet Google https://tfhub.dev/ e1 Current Biology 31, 3315–3329.e1–e5, August 9, 2021 ll ..
cAMP Assay:Article Title: Optimized Design and in Vivo Application of Optogenetically Modified Drosophila Dopamine Receptors to Elucidate Endogenous Functions
Article Snippet: .. ©@ CelPress STAR* METHODS KEY RESOURCES TABLE Neuron REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains rAAV2/18&2.CamKlla(0.4).OPN3-mScarlet This paper N/A rAAV2/1&2.CamKlla..eYFP.WPRE This paper N/A rAAV2/1&2.CamKla(0.4).eOPN3-mScarlet This paper www.addgene.org/125712/ rAAV2/1&2.hSyn.SIO-eOPN3-mScarlet This paper www.addgene.org/125713/ Chemicals, peptides, and recombinant proteins (R)-baclofen Tocris Cat#0796 Clozapine-N-Oxide Enzo Life Science Cat#-BML-NS105 CPPene Tocris Cat#1265 Gabazine Tocris Cat#1262 NBQX Tocris Cat#1044 Pertussis toxin Sigma-Aldrich Cat#516560 Picrotoxin Tocris Cat#1128 SCH23390 Tocris Cat#0925 Critical commercial assays GloSensor cAMP Assay Promega Cat#E1171 Experimental models: Cell lines HEK293T Sigma-Aldrich Cat#12022001 RRID:CVCL_0063 Experimental models: Organisms/strains Mouse: C57BL/6JRecHsd Envigo Cat#043 Mouse: C57BL/6NHsd Envigo Cat#044 Mouse: DAT-IRES-Cre The Jackson Laboratory Strain #006660 Rattus norvegicus: Sprague-Dawley Envigo Cat#002 Rattus norvegicus: Wistar Charles River, bred in the Cat#003 animal facility, UKE Hamburg Recombinant DNA pAAV-CaMkKlla(0.4)-OPN3-mScarlet This Paper N/A pAAV-CaMkKlla(0.4)-PufTMT3a-mScarlet This Paper N/A pAAV-CaMKlla(0.4)-OPN3-M4-mScarlet This Paper N/A pAAV-CaMKlla(0.4)-PufTMT3a-M4-mScarlet This Paper N/A pAAV-CamKlla-eYFP. pcDNA3.1-GIRK2-1 pcDNA3.1-mCerulean pAAV-CaMKlla(0.4)-eOPN3-mScarlet pAAV-hSyn-SIO-eOPN3-mScarlet Software and algorithms Fiji MATLAB 2018b Prism 8.2.1 RStudio Desktop Ephus. .. WaveSurfer e1 Neuron 109, 1621-1635.e1-e8, May 19, 2021 Karl Deisseroth Eitan Reuveny Dave Piston; Rizzo et al.,2004 This Paper This Paper Schindelin et al., 2012 Mathworks Graphpad RStudio Suter et al., 2010 Janelia 117 RRID:Addgene_105622; www.addgene.org/105622 GenBank: NM_001025584.2 RRID:Addgene_15214; www.addgene.org/15214/ RRID:Addgene_125712; www.addgene.org/125712/ RRID:Addgene_125713; www.addgene.org/125713/ RRID:SCR_0022885; http://imagej.net/Fiji RRID:SCR_001622; www.mathworks.com RRID:SCR_002798; https://www.graphpad.com RRID:SCR_000432; https://www.rstudio.com https://doi.org/10.3389/fncir.2010.00100 https://wavesurfer.janelia.org (Continued on next page)
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